Device
Clone in
Part:BBa_K323110
Designed by: Tina Lebar Group: iGEM10_Slovenia (2010-10-20)
pBAD_BsaI_DTER
This part is composited from the pBAD promoter (Part:BBa_I0500), a double terminator (Part:BBa_B0015) and a BsaI restriction site inbetween. The BsaI restriction endonuclease cuts the DNA outside of its recognition site. BsaI restriction site (clone in site) is designed in such a way that any BioBrick part, cut with XbaI and NotI enzymes can be ligated into this vector cut with the BsaI enzyme.
Therefore, the purpose of this part is cloning any BioBrick protein coding sequence between a promoter and a terminator in only one ligation step instead of two.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1145
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 980
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1260
Illegal BsaI.rc site found at 1236
Illegal SapI site found at 962
[edit]
Categories
Parameters
None |